Gene Expression & Regulation unit test
A test on this unit alone, marked as a percentage and a letter grade — for the test your class is actually sitting, rather than for May. Answer everything, then submit once: seeing the answer to question 3 before attempting question 4 makes the final percentage meaningless.
Operon
Lab: Restriction Enzyme Analysis
Translation
Plasmid
Homeotic genes
DNA polymerase
Topoisomerase
Origin of replication
Alternative splicing
lac operon
Promoter and TATA box
Point mutation types
Short answer 1. Define or explain: Lab: Bacterial Transformation
3 ptsShort answer 2. Define or explain: Ribosome A, P and E sites
3 ptsShort answer 3. Define or explain: Transcription factors
3 ptsShort answer 4. Define or explain: Mismatch repair and proofreading
3 ptsFree response
4 ptsA gene in a bacterium is transcribed only when lactose is present in the growth medium and glucose is absent.
Identify whether this operon is inducible or repressible, and justify your answer.
Explain the role of the repressor protein when lactose is absent.
Explain the advantage to the bacterium of regulating these genes rather than expressing them constantly.