Chemistry of Life unit test
A test on this unit alone, marked as a percentage and a letter grade — for the test your class is actually sitting, rather than for May. Answer everything, then submit once: seeing the answer to question 3 before attempting question 4 makes the final percentage meaningless.
Nucleotide structure
Buffer
Lipid structure
Carbohydrate structure and function
Denaturation
Nucleic acid directionality
Dehydration synthesis
Antiparallel strands
Peptide bond
Saturated vs unsaturated fat
Disulfide bridge
Directionality and complementarity
Short answer 1. Define or explain: Phospholipid amphipathicity
3 ptsShort answer 2. Define or explain: Carbonic acid–bicarbonate buffer
3 ptsShort answer 3. Define or explain: Surface tension
3 ptsShort answer 4. Define or explain: Monomers of the four macromolecules
3 ptsFree response
9 ptsThis course has no free-response prompt tagged to this unit, so one from elsewhere in the course is used. It is still worth writing — the skill transfers.
A student performs a floating leaf disk assay. Spinach disks are vacuum-infiltrated so they sink, placed in 0.2% sodium bicarbonate solution, and illuminated by a lamp at four distances. The time for half the disks to float (ET50) is recorded: 10 cm, 8 min; 20 cm, 15 min; 30 cm, 30 min; 40 cm, 52 min. A fifth set of disks in bicarbonate-free water at 10 cm never floated.
Explain why the disks float and identify the gas responsible.
Describe the relationship between lamp distance and ET50 and explain it in terms of light intensity.
Explain the purpose of the bicarbonate-free set and state what its result demonstrates.
Predict and justify the result of repeating the 10 cm trial in the presence of DCMU, an inhibitor of electron transfer out of photosystem II.
Explain why moving the lamp introduces a confounding variable and describe how to control it.